SrasV1, Main, Exploration, bibRecord, 002361

SARS coronavirus 8b reduces viral replication by down-regulating E via an ubiquitin-independent proteasome pathway

Identifieur interne : 002361 ( Main/Exploration ); précédent : 002360; suivant : 002362

SARS coronavirus 8b reduces viral replication by down-regulating E via an ubiquitin-independent proteasome pathway

Auteurs : Choong-Tat Keng [Singapour] ; Sara Akerström [Suède] ; Cynthia Sau-Wai Leung [Hong Kong] ; Leo L. M. Poon [Hong Kong] ; J. S. Malik Peiris [Hong Kong] ; Ali Mirazimi [Suède] ; Yee-Joo Tan [Singapour]

Source :

Microbes and infection [ 1286-4579 ] ; 2011.

RBID : Pascal:11-0108132

Descripteurs français

Pascal (Inist)
- Virus syndrome respiratoire aigu sévère, Réplication, Ubiquitine, Multicatalytic endopeptidase complex, Protéine, Syndrome respiratoire aigu sévère.

English descriptors

KwdEn :
- Multicatalytic endopeptidase complex, Protein, Replication, Severe acute respiratory syndrome, Severe acute respiratory syndrome virus, Ubiquitin.

Abstract

The severe acute respiratory syndrome coronavirus (SARS-CoV) 8b protein, which is not expressed by other known coronaviruses, can down-regulate the envelope (E) protein via a proteasome-dependent pathway. Here, we showed that the down-regulation of E is not dependent on the lysine residues on 8b and the reduction of polyubiquitination of E mutants is not correlated with their down-regulation by 8b, suggesting an ubiquitin-independent proteasome pathway is involved. A time-course study revealed that 8b was expressed at late-stages of SARS-CoV infection. By using Vero E6 cells stably expressing green fluorescence protein-tagged 8b, ectopic expression of 8b was shown to significantly reduce the production of progeny virus and down-regulate E expression. Taken together, these results suggest that 8b negatively modulates virus replication by down-regulating E via an ubiquitin-independent proteasome pathway.

Affiliations:

Links toward previous steps (curation, corpus...)

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Le document en format XML

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<series><title level="j" type="main">Microbes and infection</title>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Multicatalytic endopeptidase complex</term>
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<front><div type="abstract" xml:lang="en">The severe acute respiratory syndrome coronavirus (SARS-CoV) 8b protein, which is not expressed by other known coronaviruses, can down-regulate the envelope (E) protein via a proteasome-dependent pathway. Here, we showed that the down-regulation of E is not dependent on the lysine residues on 8b and the reduction of polyubiquitination of E mutants is not correlated with their down-regulation by 8b, suggesting an ubiquitin-independent proteasome pathway is involved. A time-course study revealed that 8b was expressed at late-stages of SARS-CoV infection. By using Vero E6 cells stably expressing green fluorescence protein-tagged 8b, ectopic expression of 8b was shown to significantly reduce the production of progeny virus and down-regulate E expression. Taken together, these results suggest that 8b negatively modulates virus replication by down-regulating E via an ubiquitin-independent proteasome pathway.</div>
</front>
</TEI>
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<name sortKey="Peiris, J S Malik" sort="Peiris, J S Malik" uniqKey="Peiris J" first="J. S. Malik" last="Peiris">J. S. Malik Peiris</name>
<name sortKey="Peiris, J S Malik" sort="Peiris, J S Malik" uniqKey="Peiris J" first="J. S. Malik" last="Peiris">J. S. Malik Peiris</name>
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SARS coronavirus 8b reduces viral replication by down-regulating E via an ubiquitin-independent proteasome pathway

SARS coronavirus 8b reduces viral replication by down-regulating E via an ubiquitin-independent proteasome pathway

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri